Fabini, who joined the Boucher lab in September 2012, has successfully defended his PhD thesis, entitled "Taxonomy of bacteria in the genomic era." After publishing 11 authored and co-authored papers (plus 4 more in the pipeline), he is off to be a Postdoc in the labs of Dr. Dominic Sauvageau (Department of Chemical and Materials Engineering) and Dr. Lisa Stein (Department of Biological Sciences) at the University of Alberta. Congrats, Fabini!
Kevin wins the Alberta Innovates Graduate Student Scholarship for his Masters degree! Congrats Kevin!
In collaboration with Dr. Munirul Alam (International Centre for Diarrheal Disease Research, Bangladesh), Yan and Tareq's new review outlining the evolution and dissemination of the pandemic lineage of Vibrio cholerae has been published. "Emergence, ecology and dispersal of the pandemic generating Vibrio cholerae lineage" is now available in the open access journal of International Microbiology.
The initial development of pandemic Vibrio cholerae highlights important gene acquisition events required for the Pandemic Generating (PG) lineage to form before being able to infect human hosts. These preliminary gene acquisition stages drive the evolution of pandemic cholera, up to the seventh and current pandemic El Tor strains. Phylogenetic analysis of PG strains indicate a replacement of El Tor predecessors that is now associated with current cholera outbreaks. Pandemic V. cholerae exists in two life stages, as a pathogen in human hosts and in environmental reservoirs, with ecological factors influencing regions that have annual patterns of infection outbreaks, such as water chemistry and host associations in environmental reservoirs. This review provides insight into the evolutionary path of PG V. cholerae, and its dispersal in the environment outside of human hosts.
Kevin Liang and Nora Hussain have been officially welcomed into the Boucher lab as Masters students! Kevin has a B.Sc. in Bioinformatics from the University of Alberta, and Nora graduated from the University of Alberta with a B.Sc. with a specialization in Microbiology. Both Kevin and Nora started off doing undergraduate research projects in the Boucher lab, and continued on to pursue graduate studies after completing their B.Sc. degrees. This will be the first group of Masters students in the Boucher lab.
Welcome back, Kevin and Nora!
Kevin and Nora are the newest graduate recruits in the Boucher lab.
After almost five years to the day, Paul successfully defended his thesis "Population dynamics of Vibrio cholerae and its close relative Vibrio metoecus in an aquatic ecosystem" and is now just a few thesis edits away from officially becoming a doctor! Paul was Yan's first grad student and is moving on to do a postdoc in the lab of Dr. Howard Ochman at the University of Texas at Austin. For the time being, he will still remain in Edmonton over the summer to wrap up various projects from his Ph.D.
In collaboration with Dr. Stefan Pukatzki (University of Colorado Denver), Dr. Daniele Provenzano (University of Texas Rio Grande Valley), and Dr. Daniel Unterweger (University of Oxford), Paul and Yan conducted an extensive study on the role of horizontal gene transfer in shaping the diversity of type VI secretion system (T6SS) loci in V. cholerae and closely related species. They discovered the existence of complex arrays of effector and immunity genes in these loci. These arrays differ even between closely related sympatric strains and appear to be formed by successive horizontal gene transfer events. The resulting accumulation of large numbers of immunity genes potentially enhance the recipient's fitness in T6SS-mediated bactericidal interactions. Additionally, they show how the accumulation of T6SS elements through horizontal gene transfer could have contributed to the evolution of some V. cholerae strains from harmless environmental bacteria to pandemic pathogens.
Yan, Fabini, and Paul collaborated with Dr. Maurizio Labbate (University Technology Sydney) and published a paper in Scientific Reports about a ~28-kb genomic island (GI; designated as GIVchS12) in a non-O1/O139 strain of Vibrio cholerae located in the same position where the Vibrio Pathogenicity Island - 1 (VPI-1) would be as it has VPI-1 site-specific recombination characteristics. VPI-1, which has so far only been found in some O1 and O139 V. cholerae strains (with pathogenic strains causing cholera epidemics and pandemics), contains the toxin-coregulated pilus (tcp) cluster. TCP is a precursor for infection of V. cholerae by the CTX phage (that contains the cholera toxin) as it serves as receptor for the phage. It is also important for the colonization of the small intestine during V. cholerae infection of the host. On the other hand, GIVchS12 does not contain the same genes as VPI-1. It contains CRISPR-Cas and type VI secretion system (T6SS) modules. CRISPR-Cas is a defense mechanism by bacteria against unwanted lateral gene transfer by recognizing foreign DNA and cleaving it. T6SS is for inter-cell anatgonism, where T6SS-harbouring bacteria produce a membrane-spanning protein complex used to puncture and kill nearby eukaryotic or prokaryotic cells.
Our survey of representative V. cholerae genomes suggests the presence of genomic islands similar to GIVchS12 containing CRISPR-Cas and T6SS modules from various strains. Natural populations of V. cholerae can serve as reservoir for diverse GIs such as GIVchS12.
Tania is awarded the 2016 Queen Elizabeth II Graduate Scholarship by the Faculty of Graduate Studies and Research. This is her second time winning this award (the first time was in 2013). Congratulations!
Tania is the recipient of the Dean's Academic Excellence Award. This award is given by the Dean of the Faculty of Science. Congratulations Tania!
Yan and Paul (here pictured in the amazing Montréal Convention Centre) safely returned from Quebec, where they attended the 16th International Symposium on Microbial Ecology hosted by the International Society for Microbial Ecology (ISME) on August 21-26, 2016. Yan and Paul presented posters titled "Relatives of Vibrio cholerae pandemic strains can be found in non-endemic areas using a novel culture-independent method" and "Mosaic evolution of the Vibrio cholerae Type VI secretion system," respectively. Both posters are projects of Paul, who has clearly been very busy.